Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis | Zendy

Xiaohong Zeng | Zendy; Hui Li | Zendy; Rongrong Zheng | Zendy; Natalia Kurepina | Zendy; Barry N. Kreiswirth | Zendy; Xilin Zhao | Zendy; Ye Xu | Zendy; Qingge Li | Zendy

Open Access

Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis

Author(s) -

Xiaohong Zeng,

Hui Li,

Rongrong Zheng,

Natalia Kurepina,

Barry N. Kreiswirth,

Xilin Zhao,

Ye Xu,

Qingge Li

Publication year - 2016

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00857-16

Subject(s) - typing , biology , mycobacterium tuberculosis , ligation , locus (genetics) , concordance , dna transposable elements , virology , genetics , dna sequencing , dna , tuberculosis , microbiology and biotechnology , genome , gene , medicine , transposable element , pathology

We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

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