Open AccessDetection and Identification of Mycobacterium spp. in Clinical Specimens by Combining the Roche Cobas Amplicor Mycobacterium tuberculosis Assay with Mycobacterium Genus Detection and Nucleic Acid Sequencing
Author(s) -
Silke Peter,
Jürg Lüthy,
Antje Voit,
Guido V. Bloemberg,
Erik C. Böttger
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00851-10
Subject(s) - mycobacterium tuberculosis , nontuberculous mycobacteria , mycobacterium , mycobacterium tuberculosis complex , tuberculosis , microbiology and biotechnology , biology , polymerase chain reaction , virology , mycobacterium fortuitum , medicine , pathology , gene , genetics
We have recently developed a PCR assay for detection of Mycobacterium spp. at the genus level based on the Cobas Amplicor platform. The sensitivities for smear-positive and smear-negative specimens were found to be 100% and 47.9%, respectively. The specificity was 97.7%, the positive predictive value 84.6%, and the negative predictive value 93.1%. In a follow-up study, we have systematically evaluated the Mycobacterium genus assay in parallel with the Cobas Amplicor Mycobacterium tuberculosis assay on 2,169 clinical specimens, including respiratory and nonrespiratory specimens. Based on the genus assay, nontuberculous mycobacteria were readily detected and identified to the species level by PCR-mediated sequencing. In addition, our data point to a limited specificity of the Cobas Amplicor M. tuberculosis assay.