Open AccessRapid and Highly Sensitive Neuraminidase Subtyping of Avian Influenza Viruses by Use of a Diagnostic DNA Microarray
Author(s) -
Astrid Gall,
Bernd Hoffmann,
Timm C. Harder,
Christian Grund,
Ralf Ehricht
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00850-09
Subject(s) - subtyping , neuraminidase , biology , influenza a virus subtype h5n1 , virology , dna microarray , influenza a virus , microarray , gene , computational biology , virus , genetics , gene expression , computer science , programming language
Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR.