Open AccessMultiplex Real-Time PCR Assay for Detection of Methicillin-Resistant Staphylococcus aureus and Associated Toxin Genes
Author(s) -
Gregory E. Fosheim,
Ainsley Nicholson,
Valérie Albrecht,
Brandi Limbago
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00795-11
Subject(s) - staphylococcus aureus , microbiology and biotechnology , toxin , multiplex , leukocidin , methicillin resistant staphylococcus aureus , toxic shock syndrome , outbreak , biology , virology , multiplex polymerase chain reaction , panton–valentine leukocidin , real time polymerase chain reaction , micrococcaceae , staphylococcal infections , anthrax toxin , polymerase chain reaction , gene , bacteria , genetics , recombinant dna , fusion protein
We describe a real-time PCR assay for the detection of methicillin-resistantStaphylococcus aureus and genes encoding toxic shock syndrome toxin 1 and Panton-Valentine leukocidin. Rapid screening and detection of toxins is a useful tool for surveillance studies and outbreak investigations involving large numbers of isolates.