Open AccessMolecular Typing of Mycobacterium abscessus Based on Tandem-Repeat Polymorphism
Author(s) -
Yan Ling Wong,
Chia Sui Ong,
Yun Fong Ngeow
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00753-12
Subject(s) - mycobacterium abscessus , typing , variable number tandem repeat , biology , nontuberculous mycobacteria , tandem repeat , genotype , locus (genetics) , genetics , mycobacterium , multilocus sequence typing , microbiology and biotechnology , virology , genome , gene , bacteria
A variable-number tandem-repeat (VNTR) typing assay for the differentiation of Mycobacterium abscessus strains was developed. This assay showed complete reproducibility, locus stability, and a discriminatory power (Hunter-Gaston discriminatory index [HGDI] of 0.9563) that is superior to that of multilocus sequencing. It is a promising tool for the investigation of Mycobacterium abscessus epidemiology and nosocomial outbreaks.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom