Lowenstein-Jensen Selective Medium for Reducing Contamination in Mycobacterium tuberculosis Culture | Zendy

Kennedy Kassaza | Zendy; Patrick Orikiriza | Zendy; Augusto E. Llosa | Zendy; Joel Bazira | Zendy; Dan Nyehangane | Zendy; AnneLaure Page | Zendy; Yap Boum | Zendy

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Lowenstein-Jensen Selective Medium for Reducing Contamination in Mycobacterium tuberculosis Culture

Author(s) -

Kennedy Kassaza,

Patrick Orikiriza,

Augusto E. Llosa,

Joel Bazira,

Dan Nyehangane,

AnneLaure Page,

Yap Boum

Publication year - 2014

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00749-14

Subject(s) - penicillin , mycobacterium tuberculosis , microbiology and biotechnology , sputum , tuberculosis , contamination , isolation (microbiology) , mycobacterium , antibiotics , biology , medicine , pathology , ecology

We compared Mycobacterium tuberculosis sputum culture recovery and contamination rates between Lowenstein-Jensen medium (LJ) containing the following decontaminants and LJ alone: (i) PANTA (n = 299), (ii) Selectatab-MB (n = 299), and (iii) penicillin G (n = 234). The contamination rate for LJ alone was approximately 31%, versus 5.0% for PANTA-containing, 2% for Selectatab-containing, and 9% for penicillin-containing media (P < 0.001). M. tuberculosis isolation rates were 9.8%, 17%, 18%, and 12% for standard LJ, PANTA, Selectatab, and penicillin cultures, respectively.

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