Open AccessComparative Analysis of Rabies Virus Reverse Transcription-PCR and Virus Isolation Using Samples from a Patient Infected with Rabies Virus
Author(s) -
Marcus Panning,
Sigrid Baumgarte,
Susanne Pfefferle,
Tanja Maier,
Andreas Martens,
Christian Drosten
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00728-10
Subject(s) - rabies virus , virology , rabies , virus , virus isolation , biology , lyssavirus , reverse transcription polymerase chain reaction , mononegavirales , isolation (microbiology) , rhabdoviridae , viral disease , microbiology and biotechnology , paramyxoviridae , gene , messenger rna , genetics
Definite and rapid diagnosis of rabies is required for individual case management as well as for public health. For the first time, a direct comparison of virus isolation with quantitative real-time reverse transcription (RT)-PCR on human rabies samples was conducted. RT-PCR was found to be more sensitive than virus isolation.