Open AccessDiagnosis of Gastric Syphilis by Direct Immunofluorescence Staining and Real-Time PCR Testing
Author(s) -
ChengYen Chen,
Kai-Hua Chi,
Robert W. George,
David L. Cox,
Amitabh Srivastava,
Mário Rui Silva,
Fátima Carneiro,
Gregory Y. Lauwers,
Ronald C. Ballard
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00721-06
Subject(s) - syphilis , staining , serology , immunofluorescence , biology , pathology , treponema , monoclonal antibody , gastric mucosa , fluorescent staining , gastric biopsy , virology , antibody , microbiology and biotechnology , medicine , helicobacter pylori , stomach , immunology , gastritis , biochemistry , human immunodeficiency virus (hiv) , genetics
We report on a case of gastric syphilis in a patient with chronic dyspepsia. The diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal DNA sequences by a real-time PCR.