Open AccessRapid Discrimination between Candida glabrata, Candida nivariensis, and Candida bracarensis by Use of a Singleplex PCR
Author(s) -
Adela Enache-Angoulvant,
Juliette Guitard,
Frédéric Grenouillet,
Tiphaine Martin,
Pascal Durrens,
Cécile Fairhead,
Christophe Hennequin
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00688-11
Subject(s) - candida glabrata , amplicon , primer (cosmetics) , biology , candida krusei , candida tropicalis , candida infections , microbiology and biotechnology , fungi imperfecti , polymerase chain reaction , gene , genetics , candida albicans , chemistry , organic chemistry
We report here a PCR-based assay using a single primer pair targeting the RPL31 gene that allows discrimination between Candida glabrata, Candida bracarensis, and Candida nivariensis according to the size of the generated amplicon.