Open AccessEvaluation of the Integrated Database Network System (IDNS) SmartGene Software for Analysis of 16S rRNA Gene Sequences for Identification of Nocardia Species
Author(s) -
Patricia S. Conville,
Patrick R. Murray,
Adrian M. Zelazny
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00681-10
Subject(s) - 16s ribosomal rna , nocardia , biology , identification (biology) , centroid , ribosomal rna , gene , computational biology , genetics , database , microbiology and biotechnology , bacteria , computer science , ecology , artificial intelligence
16S rRNA gene sequences of 102Nocardia isolates were analyzed using the Integrated Database Network System (IDNS) SmartGene centroid database. A total of 76% of the isolates were correctly identified. Discordant identifications were due to inadequate centroid length (3 species), inaccurate or insufficient entries in the public databases (5 species), and heterogeneous sequences among members of a species (1 species).