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PCR-Based Identification of Common Colonization Factor Antigens of Enterotoxigenic Escherichia coli
Author(s) -
Abhisek Ghosal,
Rudra Bhowmick,
Ranjan K. Nandy,
T. Ramamurthy,
Nabendu Sekhar Chatterjee
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00646-07
Subject(s) - enterotoxigenic escherichia coli , colonization , microbiology and biotechnology , escherichia coli , antigen , biology , identification (biology) , enterobacteriaceae , virology , immunology , enterotoxin , genetics , gene , botany
Colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli (ETEC) have been classified into several groups based on their distinct antigenicity. We describe here a PCR-based method to detect common CFAs of ETEC, which were characterized using conventional serology. This PCR assay is simple and sensitive for the detection of expressed CFA genes.

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