Culture and PCR Detection of Haemophilus influenzae and Haemophilus haemolyticus in Australian Indigenous Children with Bronchiectasis | Zendy

Kim M. Hare | Zendy; Michael Binks | Zendy; Keith Grimwood | Zendy; Anne B. Chang | Zendy; Amanda Leach | Zendy; Heidi SmithVaughan | Zendy

Open Access

Culture and PCR Detection of Haemophilus influenzae and Haemophilus haemolyticus in Australian Indigenous Children with Bronchiectasis

Author(s) -

Kim M. Hare,

Michael Binks,

Keith Grimwood,

Anne B. Chang,

Amanda Leach,

Heidi SmithVaughan

Publication year - 2012

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00566-12

Subject(s) - haemophilus influenzae , bronchiectasis , haemophilus , microbiology and biotechnology , indigenous , pasteurellaceae , polymerase chain reaction , virology , medicine , biology , bacteria , genetics , antibiotics , gene , ecology , lung

A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, only 39% of oropharyngeal specimens with phenotypic NTHI had H. influenzae. The nasopharynx is therefore the preferred site for NTHI colonization studies, and NTHI is confirmed as an important lower-airway pathogen.

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