Comparison of a Novel Real-Time PCR Assay with Sequence Analysis, Reverse Hybridization, and Multiplex PCR for Hepatitis B Virus Type B and C Genotyping | Zendy

Yao Zhao | Zendy; Xiuyu Zhang | Zendy; Yuan Hu | Zendy; Wenlu Zhang | Zendy; Jieli Hu | Zendy; Aizhong Zeng | Zendy; Jinjun Guo | Zendy; Wenxiang Huang | Zendy; Weixian Chen | Zendy; Shan Youlan | Zendy; Ailong Huang | Zendy

Open Access

Comparison of a Novel Real-Time PCR Assay with Sequence Analysis, Reverse Hybridization, and Multiplex PCR for Hepatitis B Virus Type B and C Genotyping

Author(s) -

Yao Zhao,

Xiuyu Zhang,

Yuan Hu,

Wenlu Zhang,

Jieli Hu,

Aizhong Zeng,

Jinjun Guo,

Wenxiang Huang,

Weixian Chen,

Shan Youlan,

Ailong Huang

Publication year - 2011

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00543-11

Subject(s) - genotyping , virology , multiplex , biology , multiplex polymerase chain reaction , polymerase chain reaction , hepatitis b virus , real time polymerase chain reaction , virus , microbiology and biotechnology , genotype , genetics , gene

We compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.

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