Open AccessComparison of a Novel Real-Time PCR Assay with Sequence Analysis, Reverse Hybridization, and Multiplex PCR for Hepatitis B Virus Type B and C Genotyping
Author(s) -
Yongkun Zhao,
Xiuyu Zhang,
Hao Yuan,
Wenlu Zhang,
Jieli Hu,
Ai-Zhong Zeng,
Jinsong Guo,
Wenxiang Huang,
Weixian Chen,
Shan Youlan,
Aiping Huang
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00543-11
Subject(s) - genotyping , virology , multiplex , biology , multiplex polymerase chain reaction , polymerase chain reaction , hepatitis b virus , real time polymerase chain reaction , virus , microbiology and biotechnology , genotype , genetics , gene
We compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.