Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance | Zendy

Cameron Buckley | Zendy; Ella Trembizki | Zendy; Rob Baird | Zendy; Marcus Y. Chen | Zendy; Basil Donovan | Zendy; Kevin Freeman | Zendy; Namraj Goire | Zendy; Rebecca Guy | Zendy; Monica M Lahra | Zendy; David G. Regan | Zendy; David M. Whiley | Zendy

Open Access

Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance

Author(s) -

Cameron Buckley,

Ella Trembizki,

Rob Baird,

Marcus Y. Chen,

Basil Donovan,

Kevin Freeman,

Namraj Goire,

Rebecca Guy,

Monica M Lahra,

David G. Regan,

David M. Whiley

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00540-15

Subject(s) - neisseria gonorrhoeae , microbiology and biotechnology , gonococcal infection , neisseriaceae , antimicrobial , neisseria , biology , gonorrhea , virology , antibiotics , bacteria , sexually transmitted disease , syphilis , genetics , human immunodeficiency virus (hiv)

A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.

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