McSpoligotyping, a One-Step Melting Curve Analysis-Based Protocol for Spoligotyping of Mycobacterium tuberculosis | Zendy

Xiaohong Zeng | Zendy; Ye Xu | Zendy; Yang Zhou | Zendy; Hui Li | Zendy; Rongrong Zheng | Zendy; Yaoju Tan | Zendy; Bingqi Sun | Zendy; Yanlin Zhao | Zendy; Qingge Li | Zendy

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McSpoligotyping, a One-Step Melting Curve Analysis-Based Protocol for Spoligotyping of Mycobacterium tuberculosis

Author(s) -

Xiaohong Zeng,

Ye Xu,

Yang Zhou,

Hui Li,

Rongrong Zheng,

Yaoju Tan,

Bingqi Sun,

Yanlin Zhao,

Qingge Li

Publication year - 2018

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00539-18

Subject(s) - mycobacterium tuberculosis , tuberculosis , typing , biology , genome , molecular epidemiology , population , virology , genetics , computational biology , genotype , medicine , gene , environmental health , pathology

The direct repeat (DR) region in theMycobacterium tuberculosis (MTB) genome is composed of highly polymorphic direct variant repeats, which are the basis of spacer oligonucleotide typing (spoligotyping) to study the population structure and epidemiology ofM. tuberculosis . However, the membrane hybridization-based detection format requires various post-PCR manipulations and is prone to carryover contamination, restricting its wide use in high-TB-burden and resource-limited countries.

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