Development of Poliovirus Extraction Method from Stool Extracts by Using Magnetic Nanoparticles Sensitized with Soluble Poliovirus Receptor
Author(s) -
Minetaro Arita
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00499-13
Subject(s) - poliovirus , extraction (chemistry) , chromatography , virology , receptor , magnetic nanoparticles , chemistry , microbiology and biotechnology , nanoparticle , biology , virus , materials science , biochemistry , nanotechnology
A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude biological samples.
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