A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine | Zendy

Nobuo Koizumi | Zendy; Chie Nakajima | Zendy; Tsunehito Harunari | Zendy; Tsutomu Tanikawa | Zendy; Toshihiro Tokiwa | Zendy; Eriko Uchimura | Zendy; Tokujiro Furuya | Zendy; Claro N. Mingala | Zendy; Marvin A. Villanueva | Zendy; Makoto Ohnishi | Zendy; Yasuhiko Suzuki | Zendy

Open Access

A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

Author(s) -

Nobuo Koizumi,

Chie Nakajima,

Tsunehito Harunari,

Tsutomu Tanikawa,

Toshihiro Tokiwa,

Eriko Uchimura,

Tokujiro Furuya,

Claro N. Mingala,

Marvin A. Villanueva,

Makoto Ohnishi,

Yasuhiko Suzuki

Publication year - 2012

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00481-12

Subject(s) - loop mediated isothermal amplification , leptospira , urine , microbiology and biotechnology , biology , 16s ribosomal rna , gene , leptospirosis , chromatography , chemistry , dna , virology , genetics , biochemistry

We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.

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