Detection of the glmM Gene in Helicobacter pylori Isolates with a Novel Primer by PCR | Zendy

María Guadalupe Córdova-Espinoza | Zendy; Rosa González Vazquez | Zendy; Iyari Morales Méndez | Zendy; Consuelo Ruelas Vargas | Zendy; Silvia Giono Cerezo | Zendy

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Detection of the glmM Gene in Helicobacter pylori Isolates with a Novel Primer by PCR

Author(s) -

María Guadalupe Córdova-Espinoza,

Rosa González Vazquez,

Iyari Morales Méndez,

Consuelo Ruelas Vargas,

Silvia Giono Cerezo

Publication year - 2011

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00461-10

Subject(s) - primer (cosmetics) , biology , helicobacter pylori , genetics , polymerase chain reaction , gene , chemistry , organic chemistry

A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori.

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