Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak Investigation | Zendy

Jonas M. Winchell | Zendy; Kathleen A. Thurman | Zendy; Stephanie L. Mitchell | Zendy; W. Lanier Thacker | Zendy; Barry S. Fields | Zendy

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Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak Investigation

Author(s) -

Jonas M. Winchell,

Kathleen A. Thurman,

Stephanie L. Mitchell,

W. Lanier Thacker,

Barry S. Fields

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00440-08

Subject(s) - mycoplasma pneumoniae , outbreak , biology , virology , mycoplasma , real time polymerase chain reaction , polymerase chain reaction , mycoplasmataceae , microbiology and biotechnology , mollicutes , gene , medicine , genetics , pneumonia

We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.

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