Open AccessApplication of a Peptide-Mediated Magnetic Separation-Phage Assay for Detection of Viable Mycobacterium avium subsp. paratuberculosis to Bovine Bulk Tank Milk and Feces Samples
Author(s) -
Antonio Foddai,
S. Strain,
Robert H. Whitlock,
Christopher T. Elliott,
Irene R. Grant
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00429-11
Subject(s) - paratuberculosis , feces , mycobacterium avium subsp. paratuberculosis , microbiology and biotechnology , mycobacterium , biology , virology , bovine milk , bacteria , food science , genetics
Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viableMycobacterium avium subsp.paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viableM. avium subsp.paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.