PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18 | Zendy

Arif Mohammad Tanmoy | Zendy; Senjuti Saha | Zendy; Gary L. Darmstadt | Zendy; Cynthia G. Whitney | Zendy; Samir K. Saha | Zendy

Open Access

PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18

Author(s) -

Arif Mohammad Tanmoy,

Senjuti Saha,

Gary L. Darmstadt,

Cynthia G. Whitney,

Samir K. Saha

Publication year - 2016

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00419-16

Subject(s) - serotype , streptococcus pneumoniae , biology , microbiology and biotechnology , virology , streptococcaceae , polymerase chain reaction , gene , genetics , antibiotics

Six multiplex-compatible PCR primers were designed to distinguish Streptococcus pneumoniae serotypes within serogroup 18 from culturable/nonculturable pneumococcal specimens, with no cross-reactivity with other serotypes and respiratory organisms. These primers will aid in the generation of better data on vaccine/nonvaccine serotypes in invasive and carriage pneumococcal surveillance and contribute to future vaccine formulation and impact studies.

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