Open AccessSurveillance for Reassortant Virus by Multiplex Reverse Transcription-PCR Specific for Eight Genomic Segments of Avian Influenza A H5N1 Viruses
Author(s) -
Prasert Auewarakul,
Kantima Sangsiriwut,
Kridsada Chaichoune,
Arunee Thitithanyat,
Witthawat Wiriyarat,
Taweesak Songserm,
Rasameepen Ponak-nguen,
Jarunee Prasertsopon,
Phisanu Pooruk,
Pathom Sawanpanyalert,
Parntep Ratanakorn,
Pilaipan Puthavathana
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00382-07
Subject(s) - reassortment , influenza a virus subtype h5n1 , virology , biology , virus , multiplex , influenza a virus , h5n1 genetic structure , reverse transcriptase , reverse transcription polymerase chain reaction , pandemic , gene , polymerase chain reaction , genetics , covid-19 , infectious disease (medical specialty) , medicine , gene expression , disease , pathology
Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex reverse transcription-PCR assay that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate surveillance for a virus resulting from reassortment between human influenza virus and the H5N1 virus.