Open AccessIdentification of Borrelia burgdorferi Ribosomal Protein L25 by the Phage Surface Display Method and Evaluation of the Protein's Value for Serodiagnosis
Author(s) -
Markus Mueller,
Sebastian Bunk,
Isabel Diterich,
Michael Weichel,
Carolin Rauter,
Dieter Hassler,
Corinna Hermann,
Reto Crameri,
Thomas Hartung
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00371-06
Subject(s) - borrelia burgdorferi , ribosomal protein , antigen , borrelia , biology , ribosomal rna , surface protein , phage display , spirochaetaceae , microbiology and biotechnology , antibody , virology , immunology , ribosome , biochemistry , gene , rna
The phage surface display technique was used to identifyBorrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.