Open AccessComparison of a Transplant Multiplex Viral Panel on the ICEPlex System with Real-Time PCR for Detection of Cytomegalovirus, Epstein-Barr Virus, and BK Virus in Clinical Specimens
Author(s) -
Reeti Khare,
Mark J. Espy,
Jayawant N. Mandrekar,
Matthew J. Binnicker
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00358-14
Subject(s) - multiplex , cytomegalovirus , virology , bk virus , virus , multiplex polymerase chain reaction , real time polymerase chain reaction , epstein–barr virus , herpesviridae , biology , polymerase chain reaction , viral disease , kidney transplantation , gene , kidney , biochemistry , bioinformatics , endocrinology
We compared a multiplex viral transplant panel on the ICEPlex system to real-time PCR for the detection of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and BK virus (BKV). The sensitivities of the ICEPlex were 83.3%, 95.5%, and 65.5% for the detection of CMV, EBV, and BKV, respectively. Interestingly, the multiplex assay detected dual infections in 16/280 (5.7%) samples tested.
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