Development and Evaluation of a Line Probe Assay for Rapid Identification of pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Strains | Zendy

Junichiro Sekiguchi | Zendy; Tomohiko Nakamura | Zendy; Tohru MiyoshiAkiyama | Zendy; Fumiko Kirikae | Zendy; Intetsu Kobayashi | Zendy; Ewa AugustynowiczKopeć | Zendy; Zofia Zwolska | Zendy; Koji Morita | Zendy; Toshinori Suetake | Zendy; Hiroshi Yoshida | Zendy; Seiya Kato | Zendy; Toru Mori | Zendy; Teruo Kirikae | Zendy

Open Access

Development and Evaluation of a Line Probe Assay for Rapid Identification of pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Strains

Author(s) -

Junichiro Sekiguchi,

Tomohiko Nakamura,

Tohru MiyoshiAkiyama,

Fumiko Kirikae,

Intetsu Kobayashi,

Ewa AugustynowiczKopeć,

Zofia Zwolska,

Koji Morita,

Toshinori Suetake,

Hiroshi Yoshida,

Seiya Kato,

Toru Mori,

Teruo Kirikae

Publication year - 2007

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00352-07

Subject(s) - pyrazinamide , mycobacterium tuberculosis , tuberculosis , microbiology and biotechnology , biology , drug resistance , virology , medicine , pathology

Resistance ofMycobacterium tuberculosis to pyrazinamide (PZA) derives mainly from mutations in thepncA gene. We developed a reverse hybridization-based line probe assay with oligonucleotide probes designed to detect mutations inpncA . The detection of PZA resistance was evaluated in 258 clinical isolates ofM. tuberculosis . The sensitivity and specificity of PZA resistance obtained by this new assay were both 100%, consistent with the results of conventional PZA susceptibility testing. This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use.

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