Open AccessGenotypic Prediction of Human Immunodeficiency Virus Type 1 Tropism by Use of Plasma and Peripheral Blood Mononuclear Cells in the Routine Clinical Laboratory
Author(s) -
Christian Paar,
Maria Geit,
Herbert Stekel,
Jörg Berg
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00336-11
Subject(s) - peripheral blood mononuclear cell , virology , tropism , biology , tissue tropism , genotype , population , lentivirus , concordance , virus , viral load , immunology , viral disease , medicine , in vitro , bioinformatics , gene , genetics , environmental health
We developed a sequencing assay for genotypic HIV-1 tropism determination. The assay allows examination of HIV RNA from plasma and HIV DNA from peripheral blood mononuclear cells (PBMC), including PBMC samples from patients with undetectable viral loads. Assessment of 100 pairs of plasma and PBMC samples showed a high concordance of 90%. With the limitations of population-based sequencing, the assay was found to be robust and suitable for the routine clinical laboratory.