Open AccessOptimization of Hexadecylpyridinium Chloride Decontamination for Culture of Mycobacterium avium subsp. paratuberculosis from Milk
Author(s) -
Laura K. Bradner,
Suelee RobbeAusterman,
Donald C. Beitz,
Judith R. Stabel
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00333-13
Subject(s) - paratuberculosis , human decontamination , mycobacterium , mycobacterium avium subsp. paratuberculosis , isolation (microbiology) , microbiology and biotechnology , colostrum , antibiotics , biology , chemistry , bacteria , medicine , immunology , antibody , genetics , pathology
A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the culturing protocol, and such protocols should be optimized to ensure that low concentrations of MAP in milk can be detected.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom