Open AccessPerformance of Panfungal- and Specific-PCR-Based Procedures for Etiological Diagnosis of Invasive Fungal Diseases on Tissue Biopsy Specimens with Proven Infection: a 7-Year Retrospective Analysis from a Reference Laboratory
Author(s) -
María José Buitrago,
Leticia BernalMartínez,
María Victoria Castelli,
J. L. Rodríguez-Tudela,
Manuel CuencaEstrella
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00328-14
Subject(s) - biopsy , retrospective cohort study , pathology , microbiological techniques , polymerase chain reaction , real time polymerase chain reaction , biology , medicine , microbiology and biotechnology , gene , biochemistry
A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.
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