Open AccessPerformance Assessment of the CapitalBio Mycobacterium Identification Array System for Identification of Mycobacteria
Author(s) -
Jingbo Liu,
Jun Yue,
Zhenlin Yan,
Moonsik Han,
Zhijun Han,
Lingjie Jin,
Yanlin Zhao
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00320-11
Subject(s) - mycobacterium , identification (biology) , microbiology and biotechnology , biology , mycobacterium avium complex , nontuberculous mycobacteria , mycobacterium infections , bacteria , genetics , botany
The CapitalBioMycobacterium identification microarray system is a rapid system for the detection ofMycobacterium tuberculosis . The performance of this system was assessed with 24 reference strains, 486Mycobacterium tuberculosis clinical isolates, and 40 clinical samples and then compared to the “gold standard” of DNA sequencing. The CapitalBioMycobacterium identification microarray system showed highly concordant identification results of 100% and 98.4% forMycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM), respectively. The sensitivity and specificity of the CapitalBioMycobacterium identification array for identification ofMycobacterium tuberculosis isolates were 99.6% and 100%, respectively, for direct detection and identification of clinical samples, and the overall sensitivity was 52.5%. It was 100% for sputum, 16.7% for pleural fluid, and 10% for bronchoalveolar lavage fluid, respectively. The total assay was completed in 6 h, including DNA extraction, PCR, and hybridization. The results of this study confirm the utility of this system for the rapid identification of mycobacteria and suggest that the CapitalBioMycobacterium identification array is a molecular diagnostic technique with high sensitivity and specificity that has the capacity to quickly identify most mycobacteria.