Open AccessDevelopment of a Disk Diffusion Method for Testing Moraxella catarrhalis Susceptibility Using Clinical and Laboratory Standards Institute Methods: a SENTRY Antimicrobial Surveillance Program Report
Author(s) -
Jan M. Bell,
John Turnidge,
Ronald N. Jones
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00304-09
Subject(s) - moraxella catarrhalis , broth microdilution , clinical microbiology , microbiology and biotechnology , moraxella (branhamella) catarrhalis , antimicrobial , agar diffusion test , moraxella , agar , biology , antibiotics , haemophilus influenzae , minimum inhibitory concentration , bacteria , staphylococcus aureus , genetics
Currently, there is no Clinical and Laboratory Standards Institute (CLSI) disk diffusion method for testing Moraxella catarrhalis susceptibility. We examined 318 clinical strains of M. catarrhalis obtained as part of the SENTRY (Asia-Pacific) Antimicrobial Surveillance Program, plus two ATCC strains. MICs were determined by the CLSI standard broth microdilution method, and zone diameters were determined on Mueller-Hinton agar incubated in 5% CO(2). All strains were examined for the presence of BRO-1 and BRO-2 beta-lactamases by using molecular techniques. Tentative zone diameter interpretive criteria were successfully developed for 19 antimicrobial agents, including nine beta-lactams, using current MIC interpretive criteria where available or wild-type cutoff values where no prior criteria were available. The proposed interpretive criteria were highly accurate, with <or=0.7% very major (falsely susceptible) and <or=1.0% major (falsely resistant) errors, respectively.