Open AccessA Novel High-Resolution Melt PCR Assay Discriminates Anaplasma phagocytophilum and “Candidatus Neoehrlichia mikurensis”
Author(s) -
Jürgen Krücken,
Cécile Schreiber,
Denny Maaz,
Mareen Kohn,
Janina Demeler,
Stefanie Beck,
E. Schein,
Philipp Olias,
D. Richter,
FranzRainer Matuschka,
Stefan Pachnicke,
Klemens J. Krieger,
Barbara Kohn,
Georg von SamsonHimmelstjerna
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00284-13
Subject(s) - anaplasma phagocytophilum , anaplasmataceae , ixodes ricinus , biology , ixodes , tick borne disease , virology , candidatus , anaplasma , anaplasmosis , microbiology and biotechnology , borrelia burgdorferi , bacteria , 16s ribosomal rna , tick , genetics , antibody
"Candidatus Neoehrlichia mikurensis" (Anaplasmataceae) is an emerging pathogen transmitted by Ixodes ticks. Conventional PCR and the newly developed high-resolution melt PCR were used to detect and discriminate "Candidatus Neoehrlichia mikurensis" and Anaplasma phagocytophilum. Both bacterial species were frequently found in Ixodes ricinus and Ixodes hexagonus but virtually absent from Dermacentor reticulatus. In rodents, "Candidatus N. mikurensis" was significantly more prevalent than A. phagocytophilum, whereas in cats, only A. phagocytophilum was found.
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