Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens | Zendy

Erik Munson | Zendy; Maureen Napierala | Zendy; Kimber L. Munson | Zendy; Dorothy Bilbo | Zendy; Michael A. Schulte | Zendy

Open Access

Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens

Author(s) -

Erik Munson,

Maureen Napierala,

Kimber L. Munson,

Dorothy Bilbo,

Michael A. Schulte

Publication year - 2016

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00271-16

Subject(s) - chromatography , centrifugation , lysis , extraction (chemistry) , biology , nucleic acid , escherichia coli , microbiology and biotechnology , chemistry , biochemistry , gene

As an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. ProGastro SSC and Shiga toxin-producing Escherichia coli (i.e., STEC) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. Following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column methods. Mean processing times for manual and column methods were 30.5 and 69.2 min, respectively. Concordance of investigational methods with automated extraction was ≥98.8%.

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