Open AccessNevirapine Resistance in Human Immunodeficiency Virus Type 1-Positive Infants Determined Using Dried Blood Spots Stored for Up to Six Years at Room Temperature
Author(s) -
Julie A. Nelson,
Amy M. Loftis,
Deborah Kamwendo,
Wafaie W. Fawzi,
Taha E. Taha,
Robert L. Goldenberg,
Susan A. Fiscus
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00254-09
Subject(s) - nevirapine , dried blood , virology , human immunodeficiency virus (hiv) , spots , virus , microbiology and biotechnology , biology , medicine , chemistry , viral load , chromatography , antiretroviral therapy , pathology
Dried blood spots that had been stored ambiently for 3 to 6 years lost approximately 1 log10 of human immunodeficiency virus type 1 (HIV-1) RNA, but the majority could still be genotyped for resistance. Nevirapine resistance was found in 7/16 (43.5%) HIV-1-positive HIVNET 024 infants at 4 to 6 weeks, but no resistance was found at other time points.