Optimization of a Laboratory-Developed Test Utilizing Roche Analyte-Specific Reagents for Detection of Staphylococcus aureus , Methicillin-Resistant S. aureus , and Vancomycin-Resistant Enterococcus Species | Zendy

Maitry S. Mehta | Zendy; Suzanne M. Paule | Zendy; Donna M. Hacek | Zendy; Richard B. Thomson | Zendy; Karen L. Kaul | Zendy; Lance R. Peterson | Zendy

Open Access

Optimization of a Laboratory-Developed Test Utilizing Roche Analyte-Specific Reagents for Detection of Staphylococcus aureus , Methicillin-Resistant S. aureus , and Vancomycin-Resistant Enterococcus Species

Author(s) -

Maitry S. Mehta,

Suzanne M. Paule,

Donna M. Hacek,

Richard B. Thomson,

Karen L. Kaul,

Lance R. Peterson

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00230-08

Subject(s) - staphylococcus aureus , microbiology and biotechnology , enterococcus , vancomycin , micrococcaceae , methicillin resistant staphylococcus aureus , analyte , antibiotics , staphylococcal infections , medicine , antibacterial agent , biology , chromatography , chemistry , bacteria , genetics

Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.

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