Open AccessUse of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Caspofungin Susceptibility Testing of Candida and Aspergillus Species
Author(s) -
Elena De Carolis,
Antonietta Vella,
Ada Rita Florio,
Patrizia Posteraro,
David S. Perlin,
Maurizio Sanguinetti,
Brunella Posteraro
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00224-12
Subject(s) - caspofungin , aspergillus , mass spectrometry , microbiology and biotechnology , matrix assisted laser desorption/ionization , antifungal , chromatography , chemistry , biology , voriconazole , desorption , organic chemistry , adsorption
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.
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