Open AccessDevelopment and Evaluation of a Point-of-Care Test in a Low-Resource Setting with High Rates of Chlamydia trachomatis Urogenital Infections in Fiji
Author(s) -
Deborah Dean,
Sumeetha Swaminathan,
Mike Kama,
Sophie Goemans,
Daniel Faktaufon,
Noor Alnabelseya,
Dawn P. Spelke,
Kamin Kahrizi,
Matthew Black,
Debkishore Mitra
Publication year - 2021
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00182-21
Subject(s) - chlamydia trachomatis , point of care testing , medicine , bacterial vaginosis , nucleic acid amplification tests , trichomonas vaginalis , pelvic inflammatory disease , vaginitis , chlamydia , gynecology , obstetrics , sexually transmitted disease , immunology , human immunodeficiency virus (hiv) , syphilis
Rapid and precise detection ofChlamydia trachomatis , the leading global cause of sexually transmitted infections (STI), at the point of care (POC) is required for treatment decisions to prevent transmission and sequelae, including pelvic inflammatory disease, ectopic pregnancy, tubal factor infertility, and preterm birth. We developed a rapid POC test (POCT), termed LH-POCT, which usesl oop-mediatedamp lification (LAMP) of nucleic acids, and performed a head-to-head comparison with the Cepheid Xpert CT/NG assay using clinician-collected, deidentified paired vaginal samples from a parent study that consecutively enrolled symptomatic and asymptomatic females over 18 years of age from the Ministry of Health and Medical Services Health Centers in Fiji.