Open AccessDetection of Mutations Conferring Resistance to Linezolid in Enterococcus spp. by Fluorescence In Situ Hybridization
Author(s) -
Guido Werner,
Melanie Bartel,
Nele Wellinghausen,
Andreas Essig,
Ingo Klare,
Wolfgang Witte,
Sven Poppert
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00179-07
Subject(s) - fluorescence in situ hybridization , enterococcus , biology , linezolid , in situ , in situ hybridization , microbiology and biotechnology , genetics , bacteria , gene , staphylococcus aureus , chemistry , vancomycin , gene expression , organic chemistry , chromosome
A fluorescence in situ hybridization (FISH) assay was established to detect linezolid resistance (conferred by the mutation 2576G>T in the gene coding for the 23 string of the ribosomal RNA) in enterococci. The assay was evaluated with 106Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates.