Open AccessQuantification by Real-Time PCR Assay of Staphylococcus aureus Load: a Useful Tool for Rapidly Identifying Persistent Nasal Carriers
Author(s) -
Paul O. Verhoeven,
Florence Grattard,
Anne Carricajo,
F. Lucht,
Céline Cazorla,
Olivier Garraud,
Bruno Pozzetto,
P. Berthelot
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00157-12
Subject(s) - staphylococcus aureus , microbiology and biotechnology , real time polymerase chain reaction , staphylococcal infections , biology , micrococcaceae , virology , computational biology , bacteria , genetics , gene
The Cepheid Xpert MRSA/SA nasal PCR assay was compared to culture for quantifying Staphylococcus aureus load from 104 nasal samples (r = 0.91, P < 0.0001). Using a bacterial load-based algorithm, the test was found able to predict the carrier state in 32 of 35 healthy volunteers (22 persistent and 13 nonpersistent carriers).
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