Open AccessMolecular Fingerprinting of Mycobacterium abscessus Strains in a Cohort of Pediatric Cystic Fibrosis Patients
Author(s) -
Kathryn Harris,
D. Kenna,
Cornelis Blauwendraat,
John C. Hartley,
Jane F. Turton,
Paul Aurora,
Garth Dixon
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00155-12
Subject(s) - cystic fibrosis , typing , variable number tandem repeat , mycobacterium abscessus , microbiology and biotechnology , strain (injury) , polymerase chain reaction , biology , dna profiling , nontuberculous mycobacteria , medicine , mycobacterium , genetics , genotype , bacteria , dna , gene
Forty-one Mycobacterium abscessus complex isolates from 17 pediatric cystic fibrosis (CF) patients were typed using a novel variable-number tandem repeat (VNTR) scheme and an automated repetitive-PCR (rep-PCR) system. Both VNTR and rep-PCR typing methods differentiate between members of the M. abscessus complex. The isolates from individual patients are indistinguishable, and the data strongly suggest that individual CF patients are persistently infected with one strain and also suggests that different CF patients can harbor the same strain.
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