Open AccessMultiple Nucleotide Substitutions in the Neisseria meningitidis Serogroup C ctrA Gene Cause False-Negative Detection by Real-Time PCR
Author(s) -
Francesca Cavrini,
Giovanna Liguori,
Arianna Andreoli,
Vittorio Sambri
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00103-10
Subject(s) - neisseria meningitidis , neisseriaceae , neisseria , biology , gene , nucleotide , meningococcal infections , genetics , microbiology and biotechnology , nucleic acid sequence , meningococcal disease , virology , bacterial genetics , bacteria , escherichia coli
Two strains ofNeisseria meningitidis serogroup C with disparate sequences ofctrA were isolated. The nucleotide substitutions did not alter the corresponding protein sequences, but they impeded the detection of these meningococcal isolates by real-time PCR.