Open AccessDelayed Processing of Blood Samples Influences Time to Positivity of Blood Cultures and Results of Gram Stain-Acridine Orange Leukocyte Cytospin Test
Author(s) -
Ines Schwetz,
G. Hinrichs,
Emil C. Reisinger,
G. J. Krejs,
Horst Olschewski,
Robert Krause
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00085-07
Subject(s) - acridine orange , gram staining , stain , differential staining , gram , biology , microbiology and biotechnology , staining , pathology , bacteria , medicine , antibiotics , genetics
We investigated in vitro whether storage of blood samples influences the time to positivity used for the calculation of the differential time to positivity (DTP) and the results of the Gram stain-acridine orange leukocyte Cytospin (AOLC) test. A 24-hour storage of blood samples at room temperature may lead to false-negative DTP and false-positive Gram stain-AOLC test results, whereas storage at 4°C does not.