Rapid and Accurate Identification of Candida albicans Isolates by Use of PNA FISH Flow | Zendy

Jan Trnovsky | Zendy; William G. Merz | Zendy; Phyllis DellaLatta | Zendy; Fann Wu | Zendy; Maiken Cavling Arendrup | Zendy; Henrik Stender | Zendy

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Rapid and Accurate Identification of Candida albicans Isolates by Use of PNA FISH ^Flow

Author(s) -

Jan Trnovsky,

William G. Merz,

Phyllis DellaLatta,

Fann Wu,

Maiken Cavling Arendrup,

Henrik Stender

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00030-08

Subject(s) - candida albicans , flow cytometry , fish <actinopterygii> , fluorescence in situ hybridization , identification (biology) , yeast , biology , fluorescence microscope , microbiology and biotechnology , fluorescence , chromatography , chemistry , biochemistry , physics , chromosome , gene , ecology , quantum mechanics , fishery

We developed the simple, rapid (1 h), and accurate PNA FISHFlow method for the identification ofCandida albicans . The method exploits unique in solution in situ hybridization conditions under which the cells are simultaneously fixed and hybridized. This method facilitates the accurate identification of clinical yeast isolates using two scoring techniques: flow cytometry and fluorescence microscopy.

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