Open AccessRole of Secondary Attachment Sites in Changing the Specificity of Site-Specific Recombination
Author(s) -
Edit Rutkai,
Andrea György,
László Dorgai,
Robert A. Weisberg
Publication year - 2006
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.188.9.3409-3411.2006
Subject(s) - integrases , biology , recombination , mutant , genetics , computational biology , gene
We previously proposed that lambdoid phages change their insertion specificity by adapting their integrases to sequences found in secondary attachment sites. To test this model, we quantified recombination between partners that carried sequences from secondary attachment sites catalyzed by wild-type and by mutant integrases with altered specificities. The results are consistent with the model, and indicate differential core site usage in excision and integration.