Open AccessInhibition of Bacillus subtilis scoC Expression by Multicopy senS
Author(s) -
Eiji Kawachi,
Sadanobu Abe,
Teruo Takano
Publication year - 2005
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.187.24.8526-8530.2005
Subject(s) - biology , bacillus subtilis , mutant , genetics , gene , transcription (linguistics) , microbiology and biotechnology , regulator , transcription factor , promoter , gene expression , bacteria , linguistics , philosophy
TheBacillus subtilis aprE gene, which encodes the extracellular alkaline protease, is regulated by many positive and negative transcriptional regulators. SenS is one such positive regulator consisting of 65 amino acids. We found that thesenS gene on a multicopy plasmid, pSEN24, caused an increase inaprE expression in strains carrying the upstream region ofaprE up to −340 with respect to the transcription initiation site but not in a strain carrying the region up to −299, which is within the binding site of the negative regulator ScoC (Hpr). Epistatic analysis showed that the pSEN24 effect was lost in ascoC -deleted mutant. In accordance with these results, thescoC transcription level as assayed by ascoC-lacZ fusion and Northern analysis was greatly reduced in the cells carrying pSEN24. From these results we conclude that multicopysenS enhancesaprE expression by suppressing the transcription ofscoC .