Open AccessThe Salmonella Translocated Effector SopA Is Targeted to the Mitochondria of Infected Cells
Author(s) -
Abigail N. Layton,
Philip J. Brown,
Edouard E. Galyov
Publication year - 2005
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.187.10.3565-3571.2005
Subject(s) - biology , effector , salmonella , salmonella enterica , transfection , mitochondrion , hemagglutinin (influenza) , plasmid , microbiology and biotechnology , virology , gene , bacteria , genetics
This study investigates the Salmonella effector protein SopA. We show that in Salmonella enterica serovar Dublin-infected cells, SopA(1-347) fused to two carboxy-terminal hemagglutinin tags partially colocalized with mitochondria. Transfection of eukaryotic cells with a panel of constructs encoding truncated versions of SopA identified that amino acids 100 to 347 were sufficient to target SopA to the mitochondria.