Open AccessEssentiality of clpX , but Not clpP , clpL , clpC , or clpE , in Streptococcus pneumoniae R6
Author(s) -
Gregory T. Robertson,
WaiLeung Ng,
Raymond Gilmour,
Malcolm E. Winkler
Publication year - 2003
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.185.9.2961-2966.2003
Subject(s) - biology , streptococcus pneumoniae , microbiology and biotechnology , genetics , bacteria
We show by using a regulated promoter that clpX of Streptococcus pneumoniae R6 is essential, whereas clpP, clpL, clpC, and clpE can be disrupted. The essentiality of clpX was initially missed because of duplication and rearrangement in the region of the chromosome containing clpX. Depletion of ClpX resulted in a rapid loss of viability without overt changes in cell morphology. Essentiality of clpX, but not clpP, has not been reported previously.