Open AccessPlasmid R16 ArdA Protein Preferentially Targets Restriction Activity of the Type I Restriction-Modification System Eco KI
Author(s) -
Angela T. Thomas,
William J. Brammar,
Brian M. Wilkins
Publication year - 2003
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.185.6.2022-2025.2003
Subject(s) - plasmid , biology , restriction fragment , restriction map , bacteria , microbiology and biotechnology , recombinant dna , genetics , biochemistry , dna , gene
The ArdA antirestriction protein of the IncB plasmid R16 selectively inhibited the restriction activity of EcoKI, leaving significant levels of modification activity under conditions in which restriction was almost completely prevented. The results are consistent with the hypothesis that ArdA functions in bacterial conjugation to allow an unmodified plasmid to evade restriction in the recipient bacterium and yet acquire cognate modification.