Open AccessTheStreptomyces coelicolorPolynucleotide PhosphorylaseHomologue, and Not the Putative Poly(A) Polymerase, CanPolyadenylateRNA
Author(s) -
Björn Sohlberg,
Jianqiang Huang,
Stanley N. Cohen
Publication year - 2003
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.185.24.7273-7278.2003
Subject(s) - polynucleotide phosphorylase , streptomyces coelicolor , biology , polyadenylation , polymerase , polynucleotide , biochemistry , transfer rna , microbiology and biotechnology , nucleotidyltransferase , streptomyces , genetics , rna , enzyme , gene , purine nucleoside phosphorylase , bacteria , purine
A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P. Bralley and G. H. Jones, Mol. Microbiol. 40:1155-1164, 2001). We show that this protein lacks poly(A) polymerase activity and is instead a tRNA nucleotidyltransferase that repairs CCA ends of tRNAs. In contrast, a Streptomyces coelicolor polynucleotide phosphorylase homologue that exhibits polyadenylation activity may account for the poly(A) tails found in this organism.