Open AccessOne Repeat of the Cell Wall Binding Domain Is Sufficient for Anchoring the Lactobacillus acidophilus Surface Layer Protein
Author(s) -
Elize Smit,
Peter H. Pouwels
Publication year - 2002
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.184.16.4617-4619.2002
Subject(s) - teichoic acid , lactobacillus acidophilus , biology , cell wall , cell , lactobacillus casei , biochemistry , plasma protein binding , lipoteichoic acid , microbiology and biotechnology , bacteria , peptidoglycan , genetics , probiotic , fermentation , staphylococcus aureus
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and sufficient for cell wall binding. Our data suggest that SAC anchors the S-protein to a cell wall teichoic acid.