Open AccessMicroarray Analysis of the Mycobacterium tuberculosis Transcriptional Response to the Acidic Conditions Found in Phagosomes
Author(s) -
Mark A. Fisher,
Bonnie B. Plikaytis,
Thomas M. Shinnick
Publication year - 2002
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.184.14.4025-4032.2002
Subject(s) - biology , phagosome , mycobacterium tuberculosis , nonribosomal peptide , dna microarray , microbiology and biotechnology , mycobacterium , gene , microarray analysis techniques , microarray , tuberculosis , bacteria , gene expression , biochemistry , genetics , biosynthesis , phagocytosis , medicine , pathology
We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis to low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed >1.5-fold, including many involved in fatty acid metabolism. The most highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.